Re: Colocalization analysis w/ ICA and JACoP
Posted by
Veronica Labrador Cantarero on
Jun 08, 2010; 4:30pm
URL: http://imagej.273.s1.nabble.com/Colocalization-analysis-w-ICA-and-JACoP-tp3687953p3687955.html
Hello everyone,
I have also found differences in Pearson and ICQ coefficients between JACoP and
ICA plugins. Please, have a look at the document "JACoP vs ICA" where
the same image was used with different grades of overlap.
In addition, I have found that Overlap coefficients are higher than expected,
in my opinion, after applying a threshold even if the degree of colocalization
is low.
I also compared Overlap and K coefficients obtained for several images with MS
Excel and with JACoP plugin. When no threshold was applied the results were
exactly the same but as I applied thresholds values became different (see the
document "Test 2").
We would really appreciate any help on this subject.
Here is the link to download the documentation:
http://rapidshare.com/files/396731811/Documentation.zip.htmlThank you in advance,
Veronica Labrador Cantarero
Servicio de Microscopia Optica y Confocal
Centro de Biologia Molecular Severo Ochoa
C/Nicolas Cabrera,1
Universidad Autonoma de Madrid
Madrid (Spain)
http://www.cbm.uam.es/confocal