Re: Colocalization analysis w/ ICA and JACoP

Posted by Gabriel Landini on Jun 09, 2010; 8:09am
URL: http://imagej.273.s1.nabble.com/Colocalization-analysis-w-ICA-and-JACoP-tp3687953p3687958.html

On Tuesday 08 Jun 2010  09:02:21 you wrote:
>
> http://rapidshare.com/files/396731811/Documentation.zip.html

Just one observation on something mentioned in the pdf document. You should
not apply "a posteriori" background correction and expect sensible results
because that type of procedure changes the data that you are precisely trying
to extract.
"A posteriori" methods use data from the image, not from the imaging system
alone. The same ball rolling algorithm on another image will change the image
differently depending on the image contents, despite that the background
correction that needs to applied is always the same.
You need to apply a different type of background correction by characterising
your imaging setup.

G.