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Re: Colocalization analysis w/ ICA and JACoP

Posted by sibert on Jun 02, 2010; 6:15pm
URL: http://imagej.273.s1.nabble.com/Colocalization-analysis-w-ICA-and-JACoP-tp3687953p3687962.html

Thanks,

Originally I was just playing around with different plugins using whole images, narrowing the region of interest significantly narrowed the differences reported, especially within the Mander's coefficients.  

The Pearson's coefficients and ICQ are still different between the two plugins with and without thresholds.  Notably, the ICA plugin seems to apply thresholds to these values, while thresholds are not applied by JACoP.  

I've attached a .pdf, summarizing the reports from each plugin for three different cells (yeast) and one image set where each channel is from a different cell (non-colocalized).  I've also attached the images individually.  I hope to collect images at higher magnification and resolution in z- series stacks soon.

-Bryan Sibert

colocalization.pdf
cell1_green.tif
cell1_red.tif
cell2_green.tif
cell2_red.tif
cell3_green.tif
cell3_red.tif
differentcells_green.tif
differentcells_red.tif