How to correct 32bits(RGB) images?

Posted by NatashaW on May 20, 2010; 1:22pm
URL: http://imagej.273.s1.nabble.com/How-to-correct-32bits-RGB-images-tp3688233.html

Dear all,

I’m working on group of images and I need to correct them by using the correspondent flat-field frame.
My images are 32(RGB) and I split them and worked on every channel.
For example, in green channel I divided by flat-field green frame then multiplied by the mean value for the same flat-field frame. And I have 2 questions please:
1) When I convert them to float (rgbip.convertToFloat) I got very bright images.. I don’t know if my images             were damaged during the converting and if I can still work on them and get information from the pixels values?
2) When I worked only with byte (without converting to float) I get my images in the same contrast so can I apply this method or I’m going to lose many details during division?

Thank you so much for any help,
Best wishes,