Re: Disector counting frame
Posted by
Albert Cardona on
Jun 10, 2008; 10:10pm
URL: http://imagej.273.s1.nabble.com/Disector-counting-frame-tp3688237p3688243.html
Hi Robert,
> Would it be possible to add an unbiased disector counting frame to the
> choices in the Grid plug-in? This would be very helpful in carrying
> out steriological counting of cells using the optical disector method.
> Have I missed an existing plug-in to do this?
You can do this with TrakEM2's dissector data type:
http://www.ini.uzh.ch/~acardona/trakem2_manual.html#dissectorsThe manual is a bit out of date, try "Measure" to get a results table of
counts.
The dissector data type lets you have counts across more than just two
sections; the results table will tag each identified object -- so
non-repeating tags are newly appearing objects.
The area where one does the dissector can be measure by doing a standard
ROI and seeing it's width/heigth in ImageJ's status bar. Unfortunately I
have not finished yet most of the measuring tools I'd like to have.
Please let me know if you run into any issues.
Albert
--
Albert Cardona
http://www.mcdb.ucla.edu/Research/Hartenstein/acardona