Greyscale calibration overlay

Posted by Michael Doube on May 19, 2010; 1:05pm
URL: http://imagej.273.s1.nabble.com/Greyscale-calibration-overlay-tp3688253.html

Hi all

I have a small feature request that could make use of overlays: a
greyscale calibration bar.

At the moment we make these by creating a new image of the same bit
depth as the original, say 200 x 20 pixels in size, filled with "ramp".
  Then multiply by the maximum value you want to display with Process >
Math > Multiply, and copy-paste into the original.

The advantage of this approach is that the scalebar gets the same LUT
and W&L as the original, but the disadvantages are that you can't move
the bar once pasted, it's destructive to the original and if you have a
stack or hyperstack you have to paste it into all the slices one by one.

I tried adding a ramp image as an overlay to a 32-bit image which had
the fire LUT, but the overlay was insensitive to both the underlying
image's LUT and W&L.

Michael