Re: Greyscale calibration overlay

Posted by Michael Schmid on May 19, 2010; 3:49pm
URL: http://imagej.273.s1.nabble.com/Greyscale-calibration-overlay-tp3688253p3688254.html

Hi Michael,

we have Analyze>Tools>Calibration bar; it duplicates the image image  
and adds the calibration bar. So it is not destructive to the  
original image, but it is no overlay. Anyhow, I think it is fine for  
presenting the final result, or do you need a calibration bar while  
still working with an image?

Michael
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On 19 May 2010, at 15:05, Michael Doube wrote:

> Hi all
>
> I have a small feature request that could make use of overlays: a  
> greyscale calibration bar.
>
> At the moment we make these by creating a new image of the same bit  
> depth as the original, say 200 x 20 pixels in size, filled with  
> "ramp".  Then multiply by the maximum value you want to display  
> with Process > Math > Multiply, and copy-paste into the original.
>
> The advantage of this approach is that the scalebar gets the same  
> LUT and W&L as the original, but the disadvantages are that you  
> can't move the bar once pasted, it's destructive to the original  
> and if you have a stack or hyperstack you have to paste it into all  
> the slices one by one.
>
> I tried adding a ramp image as an overlay to a 32-bit image which  
> had the fire LUT, but the overlay was insensitive to both the  
> underlying image's LUT and W&L.
>
> Michael