Re: Greyscale calibration overlay

Posted by Michael Doube on May 19, 2010; 8:49pm
URL: http://imagej.273.s1.nabble.com/Greyscale-calibration-overlay-tp3688253p3688255.html

Michael,

As you say, that feature is fine if you are creating a 2D figure for
publication, but you cannot explore the original data while
simultaneously viewing a calibration.  I have in mind in particular the
3D thickness images that result from Local Thickness.

Michael

> Hi Michael,
>
> we have Analyze>Tools>Calibration bar; it duplicates the image image
> and adds the calibration bar. So it is not destructive to the
> original image, but it is no overlay. Anyhow, I think it is fine for
> presenting the final result, or do you need a calibration bar while
> still working with an image?
>
> Michael
> ________________________________________________________________
>
> On 19 May 2010, at 15:05, Michael Doube wrote:
>
>> Hi all
>>
>> I have a small feature request that could make use of overlays: a
>> greyscale calibration bar.
>>
>> At the moment we make these by creating a new image of the same bit
>> depth as the original, say 200 x 20 pixels in size, filled with
>> "ramp".  Then multiply by the maximum value you want to display
>> with Process>  Math>  Multiply, and copy-paste into the original.
>>
>> The advantage of this approach is that the scalebar gets the same
>> LUT and W&L as the original, but the disadvantages are that you
>> can't move the bar once pasted, it's destructive to the original
>> and if you have a stack or hyperstack you have to paste it into all
>> the slices one by one.
>>
>> I tried adding a ramp image as an overlay to a 32-bit image which
>> had the fire LUT, but the overlay was insensitive to both the
>> underlying image's LUT and W&L.
>>
>> Michael

--
Dr Michael Doube  BPhil BVSc PhD MRCVS
Research Associate
Department of Bioengineering
Imperial College London
South Kensington Campus
London  SW7 2AZ
United Kingdom