Re: Need help to analyze an image

Posted by Rasband, Wayne (NIH/NIMH) [E] on May 15, 2010; 2:18am
URL: http://imagej.273.s1.nabble.com/Need-help-to-analyze-an-image-tp3688287p3688288.html

On May 14, 2010, at 6:16 AM, Nariman wrote:

> Hello,
>
> I'm new to imageJ and I need your help please !!
> I have attached my image and I want to separate this two cells, I found that
> analyze particle plugin can count the cells as i want but how i can separate
> them before using it?
>
> Thank you for any help or suggestions ..
> http://n2.nabble.com/file/n5050304/cells.jpg 

You can separate the cells using Process>Binary>Watershed but some preprocessing (blurring) is required. Here is an example macro:

  rename("RGB"); // known name needed for redirect
  run("Duplicate...", "title=Mask");
  run("8-bit");
  run("Gaussian Blur...", "sigma=2");
  setAutoThreshold("Li dark");
  run("Convert to Mask");
  run("Watershed");
  run("Set Measurements...", "area mean min limit redirect=RGB");
  run("Analyze Particles...", "size=1000-Infinity display clear");

-wayne