Re: Need help to analyze an image
Posted by Nariman on May 15, 2010; 7:06am
URL: http://imagej.273.s1.nabble.com/Need-help-to-analyze-an-image-tp3688287p3688289.html
Hello,
I'm sorry for asking again but I don't know why watershed separated the particles in this way (I'v attached my image) !!
Maybe I should detect the edges before or something else? or there is another solution??
(I applied the same macro that Wayne wrote it, I added only "Subtract Background" before it and I tried to enhance the contrast but it was always the same results!!)
Thank you so much for your help..
URL: http://imagej.273.s1.nabble.com/Need-help-to-analyze-an-image-tp3688287p3688289.html
Hello,
I'm sorry for asking again but I don't know why watershed separated the particles in this way (I'v attached my image) !!
Maybe I should detect the edges before or something else? or there is another solution??
(I applied the same macro that Wayne wrote it, I added only "Subtract Background" before it and I tried to enhance the contrast but it was always the same results!!)
Thank you so much for your help..
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