Re: Need help to analyze an image

Posted by David Webster on May 15, 2010; 11:43pm
URL: http://imagej.273.s1.nabble.com/Need-help-to-analyze-an-image-tp3688287p3688290.html

I'm confused, as usual. Are you trying to do someting like partition the two
touching cells into two separate cells using this image?

David

On Sat, May 15, 2010 at 12:06 AM, Nariman <[hidden email]> wrote:

> Hello,
> I'm sorry for asking again but I don't know why watershed separated the
> particles in this way (I'v attached my image) !!
> Maybe I should detect the edges before or something else? or there is
> another solution??
> (I applied the same macro that Wayne wrote it, I added only "Subtract
> Background" before it and I tried to enhance the contrast but it was always
> the same results!!)
> Thank you so much for your help..
> http://n2.nabble.com/file/n5058431/Mask.png
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