Re: Need help to analyze an image
Posted by
Michael Schmid on
May 16, 2010; 3:52pm
URL: http://imagej.273.s1.nabble.com/Need-help-to-analyze-an-image-tp3688287p3688291.html
> I'm sorry for asking again but I don't know why watershed separated the
> particles in this way (I'v attached my image) !!
> Maybe I should detect the edges before or something else? or there is
> another solution??
>
http://n2.nabble.com/file/n5058431/Mask.pngIt is hard to see in the png image (it seems to be downscaled) - there
seems to be a segmentation at a constriction in one of the cells, so the
standard Watershed command is too sensitive. See this post for a macro
that gives you control over the sensitivity of watershed:
https://list.nih.gov/cgi-bin/wa.exe?A2=ind0909&L=IMAGEJ&F=&S=&P=214248Michael