Re: Need help to analyze an image

Posted by Michael Schmid on May 14, 2010; 12:43pm
URL: http://imagej.273.s1.nabble.com/Need-help-to-analyze-an-image-tp3688287p3688292.html

Hi Nariman,

Probably the easiest is Process>Binary>Watershed on the thresholded  
image. Before this, it may help to run a slight background  
subtraction (Process>Subtract Background, sliding paraboloid, no  
light background, radius a few hundred).

If you want to do measurements where the binary (thresholded) image  
is insufficient, use 'Redirect to' to the original data.

Michael
________________________________________________________________

On 14 May 2010, at 12:16, Nariman wrote:

> Hello,
>
> I'm new to imageJ and I need your help please !!
> I have attached my image and I want to separate this two cells, I  
> found that
> analyze particle plugin can count the cells as i want but how i can  
> separate
> them before using it?
>
> Thank you for any help or suggestions ..
> http://n2.nabble.com/file/n5050304/cells.jpg