Posted by
Hoeppner, Daniel (NIH/NINDS) [E] on
May 14, 2010; 2:03pm
URL: http://imagej.273.s1.nabble.com/Need-help-to-analyze-an-image-tp3688287p3688293.html
Hi Nariman,
The attached macro will separate your nuclei.
I recommend using a local threshold approach as you have a range of signals from the different nuclei. You'll have to download G. Landini's Auto_Local_Threshold .jar file, place it in the plugins folder of ImageJ then restart ImageJ before running the macro. To run the macro, select plugins>macros>run. Browse for the macro and select open.
http://www.dentistry.bham.ac.uk/landinig/software/software.htmlText of the attached macro:
run("8-bit");
run("Auto Local Threshold", "method=MidGrey radius=5 parameter_1=0 parameter_2=0 white");
run("Analyze Particles...", "size=5000-Infinity circularity=0.00-1.00 show=Nothing clear summarize add");
run("Revert");
Best Wishes,
Dan Hoeppner
NIH
On 5/14/10 8:43 AM, "Michael Schmid" <
[hidden email]> wrote:
Hi Nariman,
Probably the easiest is Process>Binary>Watershed on the thresholded
image. Before this, it may help to run a slight background
subtraction (Process>Subtract Background, sliding paraboloid, no
light background, radius a few hundred).
If you want to do measurements where the binary (thresholded) image
is insufficient, use 'Redirect to' to the original data.
Michael
________________________________________________________________
On 14 May 2010, at 12:16, Nariman wrote:
> Hello,
>
> I'm new to imageJ and I need your help please !!
> I have attached my image and I want to separate this two cells, I
> found that
> analyze particle plugin can count the cells as i want but how i can
> separate
> them before using it?
>
> Thank you for any help or suggestions ..
>
http://n2.nabble.com/file/n5050304/cells.jpg