Re: Need help to analyze an image

Posted by Nariman on May 14, 2010; 3:40pm
URL: http://imagej.273.s1.nabble.com/Need-help-to-analyze-an-image-tp3688287p3688294.html

Hi Daniel and Michael,

Indeed when I tried the same macro, I got 11 particles and they It doesn't encircle the nuclei !!
(and when I applied it to my real image which is .tif I didn't get any result !!)

Then I applied the same instruction that Michael told me about:

run("Subtract Background...", "rolling=300 sliding");

Then I should apply Watershed on the thresholded image, I tried to threshold the image after applying "contrast enhance" and this didn't help me too. This is the macro that I apply it

run("Enhance Contrast", "saturated=0.4");
run("Subtract Background...", "rolling=300 sliding");
run("MultiThresholder", "isodata");
run("8-bit");
run("Watershed");

Watershed didn't segment my cells well!!

Is there any preprocessing could help me before that?
Thank you so much for your help..