vesicle distribution in cell population

Posted by CosminM on Apr 28, 2010; 9:36pm
URL: http://imagej.273.s1.nabble.com/vesicle-distribution-in-cell-population-tp3688365.html

Hi All,

I have 2 D images of double stained cells - the cell membrane and some cytoplasmic vesicles – the number of which varies from cell to cell. Each image has 10-30 cells, and both the membrane stain and the vesicle stain can be easily segmented.

I am interested to extract a distribution of the vesicle number inside the individual cells at the cell population level, and to do that in an automatic fashion. I was thinking something along the lines of generating a mask from the membrane stain, registering the position and the outline of the cell area, and then apply that information to the image containing the vesicle stain in order to count and assign the number of vesicles to each cell.

Any ideas on to how to implement this with ImageJ?

Thanks,
Cosmin