Analyze particles question..

Posted by NatashaW on May 06, 2010; 4:35pm
URL: http://imagej.273.s1.nabble.com/Analyze-particles-question-tp3688376.html

Dear all,

I have a fluorescent objects (green focis) in my image which are grouped together (every group belong to a cell) so I applied a mask from ROI manager to cluster them and I succeeded in this step.
Now my fluorescent objects are clustered in groups, and I need to count the particles in every group and give some details about them.
I should apply the Particle_Analysis plugin for every group to count them, but I should convert the image to a binary mask before applying it and in this way I'll lose the important details (size, intensity).
Maybe there are some features or methods that I don't know in imageJ to save these details?
Anyone can help me or give me an advice?

Thank you,