Posted by
Michael Schmid on
May 06, 2010; 4:53pm
URL: http://imagej.273.s1.nabble.com/Analyze-particles-question-tp3688376p3688377.html
Hi Natasha,
two remarks on this:
(1) Analyze particles is restricted to the current selection (region
of interest, roi) so if you have a roi encompassing one group you
restrict the measurements to this group.
(2) If you can't define the particles by simple thresholding (which
does not affect the image data, so you can still measure min, max,
mean intensity, etc.): Did you have a look at the 'redirect to'
feature in 'Set Measurements'? It allows you to do the measurements
on an image that is different from the one that determines the
particles. Set 'redirect to' to the image with the gray values and
run 'Anlayze particles' on the mask.
Michael
________________________________________________________________
On 6 May 2010, at 18:35, NatashaW wrote:
> Dear all,
>
> I have a fluorescent objects (green focis) in my image which are
> grouped
> together (every group belong to a cell) so I applied a mask from
> ROI manager
> to cluster them and I succeeded in this step.
> Now my fluorescent objects are clustered in groups, and I need to
> count the
> particles in every group and give some details about them.
> I should apply the Particle_Analysis plugin for every group to
> count them,
> but I should convert the image to a binary mask before applying it
> and in
> this way I'll lose the important details (size, intensity).
> Maybe there are some features or methods that I don't know in
> imageJ to save
> these details?
> Anyone can help me or give me an advice?
>
> Thank you,