Re: Analyze particles question..

Posted by Michael Schmid on May 07, 2010; 3:41pm
URL: http://imagej.273.s1.nabble.com/Analyze-particles-question-tp3688376p3688379.html

Hi Natasha,

there is an experimental version of 'Color Threshold' in ImageJ. In  
contrast to grayscale thresholding it is destructive - you can't use  
it like grayscale thresholding for analyzing particles and measuring  
the original pixel values. Anyhow, measurements of min, mix, mean  
intensity for RGB images use the gray value, so you can convert you  
image to grayscale before doing any measurements and then have  
'standard' grayscale thresholding.

'Analyze>Analyze Particles' counts thresholded particles and does  
measurements on them (depending on what you set in Analyze>Set  
Measurements. In some cases, 'Process>Find Maxima' is also useful for  
counting; use preview for finding a suitable value of the tolerance.

Michael
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On 7 May 2010, at 11:21, NatashaW wrote:

> Thank you Michael !! That's what I was looking for..
> This feature works only on gray images or also on RGB images?
>
> another question: I didn't understand if I can define the particles by
> simple thresholding, how this will help me to count them easier?