Re: Help writing a macro-with pictures!

Posted by EHB2010 on
URL: http://imagej.273.s1.nabble.com/Help-writing-a-macro-with-pictures-tp3688441p3688446.html

Hi gabrielle,

if I understand correctly you intend to define the boundary by one measurement of density and give one result  also in a measurement of density (albeit a different one). That would worry me a little as I think it would be very very critical what threshold of density was used to define the boundary.

Another thing is that pixels are rectangular, not spherical like the dots in your picture. Is each black circle in your picture supposed to be 1 pixel or a large number of pixels?

I'm afraid I've not used the find maxima function and haven't read the source code. Is the confocal image at the end of your post before or after using that function?

bw

eerke