Re: Counting particle (fluorescence labelled) using ImageJ

Posted by Sindy Kueh on
URL: http://imagej.273.s1.nabble.com/Re-Counting-particle-fluorescence-labelled-using-ImageJ-tp3688447.html

Hi all

I am a newbie with using imageJ and am trying to count the number of
clusters and also to measure the cluster size of GABAA receptor a1
subunit (fluorescence labelled). I have tried looking up on the ImageJ
homepage and could not find or do not know where to look for information
on how to correctly set threshold etc on counting these
clusters/particles. I have downloaded a 2 page pdf file which explains
how to count particle etc, but have difficulty locating the file-embryos
in which is needed to do the "tutorial".
I read that in order to perform the analysis, I have to change the file
type to either 16-bit or 8 bit-gray scale, which I did... however, I had
difficulty trying to set the threshold and have returned results in the
order of thousands of clusters where only a few hundreds are present. I
have attached an example of a file of what I need to measure... the
clusters are labelled green.
I hope someone can help.

Many thanks

Best regards
Sindy



 <<Example1.JPG>>