Re: Western blot OD analysis

Posted by Gabriel Landini on Apr 17, 2010; 10:15am
URL: http://imagej.273.s1.nabble.com/Western-blot-OD-analysis-tp3688571p3688574.html

> 1. subtract the background with rolling ball radius of 25.

Applying "a-posteriori" background correction methods on images that will be
subjected to optical density measurements seems to me a very bad idea.

Cheers
G.