Re: scale bar and profiles plotting
Posted by Rasband, Wayne (NIH/NIMH) [E] on Jan 28, 2010; 6:39pm
URL: http://imagej.273.s1.nabble.com/scale-bar-and-profiles-plotting-tp3688713p3688717.html
On Jan 28, 2010, at 1:00 PM, Gabriel Landini wrote:
> On Thursday 28 January 2010, you wrote:
>> Actually, it would Be interesting to somehow combine the scale
>> bar and the overlay function in order to create a non-destructive
>> overlay of the scale bar. Would that be feasible?
>
> And how do you save the overlay?
In the current version of ImageJ (1.43o), you can either save a flattened RGB version of the image, or move the selections in the overlay to the ROI Manager (Image>Overlay>To ROI Manager) and save them using More>>Save. In ImageJ 1.43p, overlays will be saved in the TIFF header. To flatten an image, press shift-f (Image>Overlay>Flatten). To add a selection to the overlay, press 'b' (Image>Overlay>Add Selection).
-wayne