Normalizing background and fluorescence intensity
Posted by Dawn Koh on Mar 24, 2010; 4:39am
URL: http://imagej.273.s1.nabble.com/Normalizing-background-and-fluorescence-intensity-tp3688820.html
Hi all,
I'm currently working on labeling DNA with Alexa Fluor Dye 488. In my images
I can see nuclei
with maximum fluorescence at S-phase and slight fluorescence when undergoing
DNA repair.
This is actually another method to assay for unscheduled DNA synthesis (UDS)
instead of using radioisotopes.
My problem begins when I try to compare images from different views or cover
slips.
As I try to compare nuclei at S phase from different cover slips,
I notice that the fluorescence intensity slightly stronger for one cover
slip and weaker for the other.
I need both cover slips to have the same intensity in order to compare the
nuclei undergoing DNA repair.
Does anyone know how I can normalize the images on Image J so that they can
be compared fairly?
Thanks!
Best Regards
Dawn