Rescaled Images in a 32 bit Stack

Posted by Maria Calvo-2 on Mar 09, 2010; 3:53pm
URL: http://imagej.273.s1.nabble.com/Rescaled-Images-in-a-32-bit-Stack-tp3689034.html

Hello everybody,

We have a "problem" when doing Stacks with images (32 bits) that have
diferent ranges of their pixel values . ImageJ takes one range of one image
to decide the color scale. But then, ImageJ aplies this color-scale to all
the images of the Stack, so that if there is any image that is more intense
than the first one, this last one is very saturated or otherwise it can be
almost all dark, even if this image alone (this is when is not in a stack)
looks very well.
The image appearance can be restored by threshold reseting, but then the
other images in the stack are rescaled again.

Is it because image depth is infinite (32 bits)? Can this be avoided somehow?

I hope you can help us. Thanks a lot.


Maria Calvo and Pol Solans

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Dra. Maria Calvo

Unitat de Microscòpia Confocal
Serveis Cientificotècnics-C.Casanova
Facultat de Medicina
Universitat de Barcelona- IDIBAPS
C/ Casanova 143
Barcelona 08036

Tel: [phone]/39930
Fax: [phone]
E-mail: [hidden email]
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