Re: Variation in brightness & spectral composition within a stack

Posted by Gabriel Landini on Mar 09, 2010; 9:40am
URL: http://imagej.273.s1.nabble.com/Variation-in-brightness-spectral-composition-within-a-stack-tp3689053p3689056.html

On Monday 08 Mar 2010  09:30:02 you wrote:
> - The trouble is that the ambient light (in the room in which the
> experiment was conducted) changed over the course of the day.
> - Your macro controls for intensity changes, but is it possible to
> compensate for changes in spectral composition, for each photo?
> - For example, is it possible to normalise the colour composition across
> the stack in the same way as above for the intensity?

Tom,
It will be extremely difficult to do this unless properly there is some
reference chart in the frames.  If you have those in, you could try something
like this:

http://imagejdocu.tudor.lu/doku.php?id=plugin:color:chart_white_balance:start

You can't do this with any arbitrary object in the image because of the
problem of metamerism. One needs one of these colour charts.

Cheers

G