Posted by
Luke Goodsell on
Feb 24, 2010; 3:46pm
URL: http://imagej.273.s1.nabble.com/Adjust-resolution-of-plot-lanes-command-tp3689205p3689207.html
Hi Herbie, thanks for your response.
The 'resolution' I'm referring to is that of the graph images produced
by the 'plot lanes' operation. The width is the same as that of
selection height on my image, which is fine, but the height is a
constant value with the height of the peaks scaled according to the
highest peak. However, I'm using chemiluminescence and a 16bit tif, so
my peak heights can vary considerably. When I try to quantify a small
peak, I cannot accurately draw the baseline on the peak as it is only
a couple of pixels high. Zooming in on the graph just magnifies the
pixels of the graph, offering no increase in accuracy.
I would like to be able to increase the (seemingly hard-coded) height
of the graphs produced to be more useful for 16 bit images. Is this
possible?
Many thanks,
Luke
On Wed, Feb 24, 2010 at 2:47 PM, Gluender <
[hidden email]> wrote:
> Luke,
>
> not quite sure what the real problem is...
>
> First, there is the spatial resolution of your images.
> This resolution will determine most of your post-processing. If it is to
> low, then you have to increase it by scanning/capturing parts.
> Do you scan the lanes or do you use a camera?
>
> Second, there is the spatial resolution of your profile graphs (x-axis).
> This resolution at best can be the same as the spatial resolution of your
> images.
> It may however be smaller. You may change it in Menu-Item "Profile Plot
> Options".
>
> Finally there is the amplitude (gray-value) resolution of your images as
> well as your profile plots (y-axis). 8 bits means 255 values different zero
> and under all circumstances your profile graph can't show more. Plot
> amplitude resolution may also be changed in Menu-Item "Profile Plot
> Options".
>
> That said you may give us a little more information about your desires.
>
>> Hi,
>>
>> I am trying to quantify my westerns but am having difficulty because
>> the resolution of the graphs output by the "plot lanes" command is too
>> low. I have a number of bands of a wide range of concentrations so the
>> smallest bands have small peaks in the graphs, making it impossible to
>> place the baseline accurately. I assumed the zoom tool would help, but
>> that just, err, makes the pixels bigger - sorry, I don't know how to
>> describe it. I also tried using multiple selections in the same lane,
>> but then the areas of the graphs are not comparable because they have
>> been scaled differently. I've attached an example of the graphs I get.
>>
>> I searched Google and the mailing list archives, and I saw at least
>> one person mention this as a side issue, but no one addressed it.
>>
>> Is it possible to change the resolution/size of the graphs output by
>> the "plot lanes" command so that I can zoom in and accurately define
>> the baseline?
>>
>> Many thanks,
>> Luke
>>
>> Content-Type: image/png; name="Picture 1.png"
>> Content-Disposition: attachment; filename="Picture 1.png"
>> X-Attachment-Id: f_g627gugk0
>>
>> Attachment converted: Download:Picture 1 3.png (PNGf/«IC») (0001832A)
>
> Best
> --
>
> Herbie
>
> ------------------------
> <
http://www.gluender.de>
>