http://imagej.273.s1.nabble.com/Adjust-resolution-of-plot-lanes-command-tp3689205p3689212.html
but it should be based on profile plots. That
plot window. In your case set Height to perhaps
>Hi Herbie, thanks for your response.
>
>The 'resolution' I'm referring to is that of the graph images produced
>by the 'plot lanes' operation. The width is the same as that of
>selection height on my image, which is fine, but the height is a
>constant value with the height of the peaks scaled according to the
>highest peak. However, I'm using chemiluminescence and a 16bit tif, so
>my peak heights can vary considerably. When I try to quantify a small
>peak, I cannot accurately draw the baseline on the peak as it is only
>a couple of pixels high. Zooming in on the graph just magnifies the
>pixels of the graph, offering no increase in accuracy.
>
>I would like to be able to increase the (seemingly hard-coded) height
>of the graphs produced to be more useful for 16 bit images. Is this
>possible?
>
>Many thanks,
>Luke
>
>On Wed, Feb 24, 2010 at 2:47 PM, Gluender <
[hidden email]> wrote:
>> Luke,
>>
>> not quite sure what the real problem is...
>>
>> First, there is the spatial resolution of your images.
>> This resolution will determine most of your post-processing. If it is to
>> low, then you have to increase it by scanning/capturing parts.
>> Do you scan the lanes or do you use a camera?
>>
>> Second, there is the spatial resolution of your profile graphs (x-axis).
>> This resolution at best can be the same as the spatial resolution of your
>> images.
>> It may however be smaller. You may change it in Menu-Item "Profile Plot
>> Options".
>>
>> Finally there is the amplitude (gray-value) resolution of your images as
>> well as your profile plots (y-axis). 8 bits means 255 values different zero
>> and under all circumstances your profile graph can't show more. Plot
>> amplitude resolution may also be changed in Menu-Item "Profile Plot
>> Options".
>>
>> That said you may give us a little more information about your desires.
>>
>>> Hi,
>>>
>>> I am trying to quantify my westerns but am having difficulty because
>>> the resolution of the graphs output by the "plot lanes" command is too
>>> low. I have a number of bands of a wide range of concentrations so the
>>> smallest bands have small peaks in the graphs, making it impossible to
>>> place the baseline accurately. I assumed the zoom tool would help, but
>>> that just, err, makes the pixels bigger - sorry, I don't know how to
>>> describe it. I also tried using multiple selections in the same lane,
>>> but then the areas of the graphs are not comparable because they have
>>> been scaled differently. I've attached an example of the graphs I get.
>>>
>>> I searched Google and the mailing list archives, and I saw at least
>>> one person mention this as a side issue, but no one addressed it.
>>>
>>> Is it possible to change the resolution/size of the graphs output by
>>> the "plot lanes" command so that I can zoom in and accurately define
>>> the baseline?
>>>
>>> Many thanks,
>>> Luke
>>>
>>> Content-Type: image/png; name="Picture 1.png"
>>> Content-Disposition: attachment; filename="Picture 1.png"
>>> X-Attachment-Id: f_g627gugk0
>>>
>>> Attachment converted: Download:Picture 1 3.png (PNGf/«IC») (0001832A)
>>
>> Best
>> --
>>
>> Herbie
>>
>> ------------------------
>> <
http://www.gluender.de>
>>