Posted by
Gluender-3 on
Feb 24, 2010; 2:47pm
URL: http://imagej.273.s1.nabble.com/Adjust-resolution-of-plot-lanes-command-tp3689205p3689213.html
Luke,
not quite sure what the real problem is...
First, there is the spatial resolution of your images.
This resolution will determine most of your
post-processing. If it is to low, then you have
to increase it by scanning/capturing parts.
Do you scan the lanes or do you use a camera?
Second, there is the spatial resolution of your profile graphs (x-axis).
This resolution at best can be the same as the
spatial resolution of your images.
It may however be smaller. You may change it in
Menu-Item "Profile Plot Options".
Finally there is the amplitude (gray-value)
resolution of your images as well as your profile
plots (y-axis). 8 bits means 255 values different
zero and under all circumstances your profile
graph can't show more. Plot amplitude resolution
may also be changed in Menu-Item "Profile Plot
Options".
That said you may give us a little more information about your desires.
>Hi,
>
>I am trying to quantify my westerns but am having difficulty because
>the resolution of the graphs output by the "plot lanes" command is too
>low. I have a number of bands of a wide range of concentrations so the
>smallest bands have small peaks in the graphs, making it impossible to
>place the baseline accurately. I assumed the zoom tool would help, but
>that just, err, makes the pixels bigger - sorry, I don't know how to
>describe it. I also tried using multiple selections in the same lane,
>but then the areas of the graphs are not comparable because they have
>been scaled differently. I've attached an example of the graphs I get.
>
>I searched Google and the mailing list archives, and I saw at least
>one person mention this as a side issue, but no one addressed it.
>
>Is it possible to change the resolution/size of the graphs output by
>the "plot lanes" command so that I can zoom in and accurately define
>the baseline?
>
>Many thanks,
>Luke
>
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>Attachment converted: Download:Picture 1 3.png (PNGf/«IC») (0001832A)
Best
--
Herbie
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