Segmentation of oligodendrocyte (cell) staining - DAB

Posted by Jacqueline Ross on Feb 11, 2010; 5:43am
URL: http://imagej.273.s1.nabble.com/Segmentation-of-oligodendrocyte-cell-staining-DAB-tp3689428.html

Dear All,

 

One of the graduate students here needs to count the number of
oligodendrocytes stained in his sections. The staining is
immunohistochemistry with DAB substrate.

 

This labelling not only stains the area around the cell but also the
cell processes. However, he just needs to know how many cells are
stained not the area occupied by fibres, etc.

 

He has a large number of images to analyse so we have been trying to see
if we can segment out the cell body staining which looks like a ring
around the cell in order to then batch-process.

 

I have tried a few things with varied success and would really value
some assistance on this. At the moment, my conclusion is that some
processing to enhance the image with subsequent manual counting may be
the only solution.

 

I have put 3 examples on our website here:
http://www.fmhs.auckland.ac.nz/sms/biru/links/imagej.aspx which vary
from easy to hard!

 

There are 3 cropped images with the full sized image also available to
download below. The images do look blue (probably lamp temperature
issue) but the staining is reasonably clear.

 

I have put arrowheads on one of the images to indicate what the student
needs to measure.

 

All suggestions welcome!

 

Kind regards,

 

Jacqui

 

Jacqueline Ross

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/
<http://www.fmhs.auckland.ac.nz/sms/biru/>