Re: Intensity analysis for ROS imaging

Posted by daschneider9 on
URL: http://imagej.273.s1.nabble.com/Intensity-analysis-for-ROS-imaging-tp3689476p3689477.html

Sougat,

"Me, too". I, too, am a relatively new user that's not a programmer but very interested in doing a correct/legitimate job at fluorescence densitometry in tissue slice. I look forward to seeing what advice the experts here can give.

Thanks for your question.
Dave

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Sougat Misra
Sent: Friday, January 29, 2010 8:28 AM
To: [hidden email]
Subject: Intensity analysis for ROS imaging

Hi Dear,

I did some confocal microscopy for ROS (Reactive oxygen species) imaging in
hepatocytes using Zeiss LSM 410. I want to measure the fluorescence intensity
from each cell. However, I don't know how to do it using ImajeJ. If anyone can
help me in this regard, it will be great.

Since I am a new user, I apologize for asking such a simple question. However,
your help will be highly appreciated.

Thanking you,

Sougat Misra
Ph.D. Candidate
University of Saskatchewan