Posted by
Li-Ping Yuan on
URL: http://imagej.273.s1.nabble.com/Intensity-analysis-for-ROS-imaging-tp3689476p3689478.html
At a very basic level, mous
Need more descriptions of what you want?
At a very basic level, mouse over cell pixels and read the intensity from the menu bar. You probably want more... an intensity profile along a straight line? an intensity histogram (and statistics) of a ROI? or some more complicated?
Li-Ping (Li) Yuan
________________________________
From: "Schneider, David A." <
[hidden email]>
To:
[hidden email]
Sent: Fri, January 29, 2010 10:45:17 AM
Subject: Re: Intensity analysis for ROS imaging
Sougat,
"Me, too". I, too, am a relatively new user that's not a programmer but very interested in doing a correct/legitimate job at fluorescence densitometry in tissue slice. I look forward to seeing what advice the experts here can give.
Thanks for your question.
Dave
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Sougat Misra
Sent: Friday, January 29, 2010 8:28 AM
To:
[hidden email]
Subject: Intensity analysis for ROS imaging
Hi Dear,
I did some confocal microscopy for ROS (Reactive oxygen species) imaging in
hepatocytes using Zeiss LSM 410. I want to measure the fluorescence intensity
from each cell. However, I don't know how to do it using ImajeJ. If anyone can
help me in this regard, it will be great.
Since I am a new user, I apologize for asking such a simple question. However,
your help will be highly appreciated.
Thanking you,
Sougat Misra
Ph.D. Candidate
University of Saskatchewan