Counting question

Posted by Konstantin B on Feb 04, 2010; 1:30am
URL: http://imagej.273.s1.nabble.com/Counting-question-tp3689510.html

Hi, I am new to imagej  and in need of help(actually I'm using fiji if this makes a difference). I'm trying to separate the blue dots from the green clusters in the attached picture and count each. So far I've figured out how to split the color, channels and have attempted to blur the clusters together by messing with the Gaussian blur and then finding edges/ sharpening until I get blobs. This works pretty well for whats in the center, but the clusters in the periphery are stretched out and I'm not sure how to include them, is this going to be possible?.Also, I'm having trouble figuring out appropriate parameters in terms of pixel size for the actual counting.

Thanks in advance,
Konstantin