Posted by
Adam Waite on
Jan 29, 2010; 5:07am
URL: http://imagej.273.s1.nabble.com/Re-Find-Maxima-Segmented-Particles-tp3689547.html
Hi Michael,
I'm afraid I've been experiencing the same problem as Dani.
I have been processing my grayscale images to make the background as uniform
as possible. Then I would run "Find Maxima" with segmented particles as the
output. Finally, by running "Analyze Particles", I could make accurate
selections around my regions of interest (cells, to be specific). This
would allow me to obtain size, shape, and intensity information about the
selection.
With the current implementation, I get what looks like a voroni diagram,
which obviously gets rid of anything approaching accurate size information.
I would like to restore the old behavior and tried to follow your advice
(quoted below), but when I go to "Find Maxima", I do not see a checkbox for
"Above Lower Threshold". Is this check box located somewhere else?
Additionally, I do not see a place where I can enter the lower or upper
threshold.
I am currently running the most recent version of ImageJ (1.43o).
Thanks in advance for your time and help,
Adam
On Sun, 15 Nov 2009 17:52:21 +0100, Michael Schmid <
[hidden email]>
wrote:
>You can restore the old behavior by setting the lower threshold to the
>background value plus one (don't care about the upper threshold, it can
>be, e.g. the maximum pixel value or the same as the lower threshold) and
>checking "Above Lower Threshold". In case your particles are minima of the
>pixel value, not maxima, you have to invert the image before "Find
>Maxima".