Posted by
Marko Usaj on
Jan 29, 2010; 9:01am
URL: http://imagej.273.s1.nabble.com/overlaying-images-tp3689556p3689558.html
I have another questions about image overlaying.
I wondering how you solve the issues of uneven staining of object of interest. The problem occurs, when you try to detect by visual inspection an overlaying colors (on the same object) but one color has much higher gray scale values than other one what has for consequence wrong interpretation.
Ok, one option is to threshold images, but I don't want to lose all dynamic range of fluorescent images. Other options is histogram equalization (BTW which function is preferred for this task in imageJ?).
Is here any other (more nobel) solution and you are willing to share with me (us)?
Best regards,
Marko
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of John Oreopoulos
Sent: Friday, January 29, 2010 4:52 AM
To:
[hidden email]
Subject: Re: overlaying images
http://rsbweb.nih.gov/ij/docs/menus/image.html#colorRGB Merge:
Merges 1-4 greyscale images or stacks into an RGB image or stack.
Select *None* to keep a channel empty (filled with 0). Check "Create
Composite" to convert 2-4 grayscale images or stacks into a composite
image or hyperstack. Check "Keep Source Images" if you wish to keep
the originals.
On 28-Jan-10, at 10:12 PM, Ramon Cajal wrote:
> Does anybody knows who to overlay 2 different images? I have 2
> images of the same object stained with two different fluorochromes
> and I need to overlay them
>
> Thanks a lot
> Ramon