Re: overlaying images
Posted by
Janne Hyötylä on
Jan 29, 2010; 10:13am
URL: http://imagej.273.s1.nabble.com/overlaying-images-tp3689556p3689559.html
Hi,
You could try using quantitative colocalization analysis:
http://www.macbiophotonics.ca/imagej/colour_analysis.htmhttp://pacific.mpi-cbg.de/wiki/index.php/ColocalizationAnalysisCheers,
Janne
On Fri, 29 Jan 2010 10:01:49 +0100, Ušaj, Marko <
[hidden email]>
wrote:
> I have another questions about image overlaying.
> I wondering how you solve the issues of uneven staining of object of
> interest. The problem occurs, when you try to detect by visual
> inspection an overlaying colors (on the same object) but one color has
> much higher gray scale values than other one what has for consequence
> wrong interpretation.
>
> Ok, one option is to threshold images, but I don't want to lose all
> dynamic range of fluorescent images. Other options is histogram
> equalization (BTW which function is preferred for this task in imageJ?).
> Is here any other (more nobel) solution and you are willing to share
> with me (us)?
>
> Best regards,
> Marko