differences in integrated densities

Posted by Karen Kempler on Jan 22, 2010; 7:44pm
URL: http://imagej.273.s1.nabble.com/differences-in-integrated-densities-tp3689600.html

  I have a problem with integrated density numbers.  I have a 75 fold difference in integrated
densities of an image depending on whether we analyze it in Image J or with the SP2 or SP5 Leica software.  We don't understand where this difference is coming from.  The areas are the same, but the densities within these areas are 75 fold less with Image J.  The problem is that we want to compile data that has already been analyzed with the SP2 software with data we are now analyzing with Image J.  Does anyone have any idea why this is?  Thank you.