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Re: differences in integrated densities

Posted by vischer on Jan 22, 2010; 9:38pm
URL: http://imagej.273.s1.nabble.com/differences-in-integrated-densities-tp3689600p3689601.html

Hi Karen,
I also had this problem until I realized that in ImageJ's equation
        IntDens=area * mean
area is scaled (e.g in square microns). Many other packages use pixel count for area. The advantage in ImageJ is that you don't get those large numbers. Personally I would have preferred the non-scaled version; when solving image processing problems, I usually think in pixels and not in microns.

regards, Norbert



>
> I have a problem with integrated density numbers.  I have a 75 fold difference in integrated
> densities of an image depending on whether we analyze it in Image J or with the SP2 or SP5 Leica software. We don't understand where this difference is coming from.  The areas are the same, but the densities within these areas are 75 fold less with Image J.  The problem is that we want to compile data that has already been analyzed with the SP2 software with data we are now analyzing with Image J.  Does anyone have any idea why this is?  Thank you.