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Color Merge Multiple Fluorescence Images

Posted by Breukers, C (TNW) on Jan 21, 2010; 8:58am
URL: http://imagej.273.s1.nabble.com/Color-Merge-Multiple-Fluorescence-Images-tp3689609.html

Dear all,

 

I'm working on a fluorescence microscope that has four fluorescence
channels (DAPI, FITC, PE, APC).

 

Since we are measuring antibody conjugated fluorescent cells, we have a
large variation in intensity and background.

 

What would be the correct way to have a color merge of four fluorescence
microscope images / channels?

What pre processing steps are necessary to modify the images such that a
nice clear color overlay is created?

 

Kind regards,

Christian Breukers

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University of Twente

Faculty of Sciences and Technology

MIRA Institute for Biomedical Technology and Technical Medicine

Department of Medical Cell BioPhysics
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