Re: basics steps for deconvolution of microscopic images

Posted by Kolb, Thorsten on Jan 08, 2010; 10:13am
URL: http://imagej.273.s1.nabble.com/basics-steps-for-deconvolution-of-microscopic-images-tp3689784p3689786.html

I do not have a point spread function, but have optical transfer
funcions from the deltavision software.
Is it possible for a non mathematician  like me to convert them to a
PSF, or can I use the OTFs
somehow directly?


Thorsten

> Dear Thorsten,
>
> If you already have a point spread function (or you can generate one),
> then my plugins should work:
>
> http://sites.google.com/site/piotrwendykier/software/deconvolution/parallelspectraldeconvolution
> http://sites.google.com/site/piotrwendykier/software/deconvolution/paralleliterativedeconvolution
>
> User guides are also available.
>
> Piotr
>
> On Thu, Jan 7, 2010 at 8:59 AM, Thorsten Kolb <[hidden email]> wrote:
>  
>> Dear all and a happy new year!
>>
>> I am working with deltavision files that I want to deconvolve with ImageJ.
>> Is there some easy to understand step by step tutorial? I searched a bit
>> with google but had no success ... Maybe someone of you can provide me the
>> necessary informations.
>>
>> Thanks a lot in advance,
>>
>> Thorsten
>>
>>    

--
Thorsten Kolb
Functional Architecture of the Cell (B065)
German Cancer Research Center (DKFZ)
Im Neuenheimer Feld 580 (TP3)
69120 Heidelberg
Germany
 
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Email: [hidden email]