Login  Register

Re: basics steps for deconvolution of microscopic images

Posted by Joel Sheffield on Jan 08, 2010; 11:14pm
URL: http://imagej.273.s1.nabble.com/basics-steps-for-deconvolution-of-microscopic-images-tp3689784p3689789.html

You might take a look at Robert Dougherty's deconvolution programs

On Fri, Jan 8, 2010 at 5:13 AM, Thorsten Kolb <[hidden email]>wrote:

> I do not have a point spread function, but have optical transfer funcions
> from the deltavision software.
> Is it possible for a non mathematician  like me to convert them to a PSF,
> or can I use the OTFs
> somehow directly?
>
>
> Thorsten
>
>  Dear Thorsten,
>>
>> If you already have a point spread function (or you can generate one),
>> then my plugins should work:
>>
>>
>> http://sites.google.com/site/piotrwendykier/software/deconvolution/parallelspectraldeconvolution
>>
>> http://sites.google.com/site/piotrwendykier/software/deconvolution/paralleliterativedeconvolution
>>
>> User guides are also available.
>>
>> Piotr
>>
>> On Thu, Jan 7, 2010 at 8:59 AM, Thorsten Kolb <[hidden email]>
>> wrote:
>>
>>
>>> Dear all and a happy new year!
>>>
>>> I am working with deltavision files that I want to deconvolve with
>>> ImageJ.
>>> Is there some easy to understand step by step tutorial? I searched a bit
>>> with google but had no success ... Maybe someone of you can provide me
>>> the
>>> necessary informations.
>>>
>>> Thanks a lot in advance,
>>>
>>> Thorsten
>>>
>>>
>>>
>>
>
> --
> Thorsten Kolb
> Functional Architecture of the Cell (B065)
> German Cancer Research Center (DKFZ)
> Im Neuenheimer Feld 580 (TP3)
> 69120 Heidelberg
> Germany
>
> Fon: +49 6221 42 3514 or 3517
> Email: [hidden email]
>



--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs <http://astro.temple.edu/%7Ejbs>