Re: Segmentation

Posted by Michael Schmid on Jan 07, 2010; 4:41pm
URL: http://imagej.273.s1.nabble.com/Segmentation-tp3689795p3689800.html

Hi Sylma (Marie?),

you can use the standard thresholding tool if you subtract the  
background.
E.g.
   run("8-bit");
   run("Subtract Background...", "rolling=500 light sliding");
   setThreshold(0, 240);

For removing residual noise from the result, you can use, e.g.,  
"Analyze Particles" with "Mask" output and a certain minimum size of  
the background objects. An alternative is
   run("Minimum...", "radius=1.5");
   run("Maximum...", "radius=1.5");

Finally, invert the images to get the bright objects. If required,  
use a further "Minimum, Maximum" sequence for smoother borders.

Michael
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On 7 Jan 2010, at 09:31, Sylma wrote:

>
> Hello
>
> I send you an image to solve my problem : lipid vesicles are white
>
> Best regards<1.jpg>