Posted by
Maciej Rudzinski on
Dec 22, 2009; 4:33am
URL: http://imagej.273.s1.nabble.com/Threshold-on-interpolated-zoomed-view-tp3689957p3689959.html
Michael,
Thank you for your inputs.
Regards,
Maciej R.
----- Original Message -----
From: "Michael Schmid" <
[hidden email]>
To:
[hidden email]
Sent: Sunday, December 20, 2009 12:30:07 PM GMT -08:00 US/Canada Pacific
Subject: Re: Threshold on interpolated zoomed view
Hi Maciej,
in my view there is no way to improve this:
If you zoom in, interpolation is only for display, but the image data are
still the original pixels. If you threshold these data, a pixel can be
only red or gray. You can't have a pixel that is partially thresholded.
For all subsequent operations, such as 'Analyze Particles', a pixel can be
inside the thresholded area or outside, nothing in between. There is no
way to have an information like "The upper 1/5 of the pixel are
thresholded". After all, a pixel is by definition the smallest entity in
an image.
So your solution of Adjust Size with interpolation is the only way to get
what you want.
Michael
_______________________________________________________________________
On Sat, December 19, 2009 17:59, Maciej Rudzinski wrote:
> Hello everyone,
>
> During image review at high magnification I need to apply threshold. At
> the time I have "interpolate zoomed images" option turned on. The
> threshold seems to be working off the original non-interpolated image
> rather than the current view. To work around I re-size the images and then
> apply the threshold but this is very inconvenient.
>
>
> I'd appreciate any input or ideas on how to solve this with a macro or any
> other way.
>
> Regards,
>
> Maciej Rudzinski
>