Rolling Ball

Posted by Breukers, C (TNW) on Dec 11, 2009; 9:31am
URL: http://imagej.273.s1.nabble.com/Rolling-Ball-tp3690109.html

Hello,

 

I have an image with fluorescence cells on a dark background.

Why are the cells (signal) still present even if I set the rolling ball
diameter much smaller than my typical cell diameter?

 

This happens even when I set the diameter to 1 pixel (my cell is more
than 4 pixels in diameter).

And how come that you can set the rolling ball diameter to even 0.001
pixel?

 

You would expect that the complete signal will be subtracted completely
resulting in a value of 0, so a complete dark image.

 

Regards,

Christian

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Faculty of Sciences and Technology

MIRA Institute for Biomedical Technology and Technical Medicine

Department of Medical Cell BioPhysics
The Netherlands