Re: automating segmentation counting of synaptic vessicles in TEM

Posted by dpoburko on Dec 10, 2009; 1:21am
URL: http://imagej.273.s1.nabble.com/Re-automating-segmentation-counting-of-synaptic-vessicles-in-TEM-tp3690149.html

Hi All,

   A collegue has asked for help in automating the counting of synaptic
vesicles in TEM images. I was wondering if anyone had any suggestions
for effective means of going about this. I can get pretty clear outlines
of the vessicles with a simple bandwidth FFT, passing the width of the
vesicle membrane. But from there I'm a little stumped as to who to
segment what effectively is a bunch of dark circles with wholes in the
middle. Any suggestions would be appreciated.

Tx,
Damon

--

Damon Poburko, PhD
Postdoctoral Research Fellow
Stanford University School of Medicine
Dept. of Molecular & Cellular Physiology
279 Campus Dr., Beckman B103, Stanford, CA 94305
Ph: 650 725 7564, fax: [phone]