how to stop the freehand lines tool at a set distance (20 um)

Posted by Matiar Jafari on Dec 06, 2009; 12:02pm
URL: http://imagej.273.s1.nabble.com/how-to-stop-the-freehand-lines-tool-at-a-set-distance-20-um-tp3690197.html

Dear ImageJ Listserv,

I'm looking for a way to draw lines of a set length on ImageJ.  I need
to measure spines on dendrites every 20 um from the soma on
dissociated neurons grown in tissue culture.  I've tried using a
modified Sholl analysis, but without the laminar constraints the
dendrites grow with various curves.  I would like to use the freehand
lines tool to stop at 20 um as I trace along the dendrite.

Thank You
----
Matiar

ps sorry if its posted multiple times im still new to this listserv and how it works