NIS Elements software

Posted by Martin Kann on Dec 04, 2009; 8:57pm
URL: http://imagej.273.s1.nabble.com/NIS-Elements-software-tp3690220.html

Hi everybody,

we are having an issue with the Nikon NIS Elements software (basic research package, version 3.00 build 550) controlling our fluorescent microscope camera. This is what happens: when taking multichannel images the red and blue channel of a .tiff image are switched if one opens this image with any other software than NIS elements. When looking into the .tiff file metadata, it appears that the TRITC image plane has been assigned blue color, and the DAPI plane red. Of course there are workarounds by using ImageJ or batch processing of files with the channel-mixer in photoshop. However, it annoys me that I have to touch each and every file one more time than necessary for my own work and frequently explain the workarounds to other people using the microscope. Has anyone ever come across this problem and knows a way to fix this in NIS elements' preferences? Maybe somebody even has a small piece of code (either Mac or PC) or a photoshop droplet that automates the task?
I would very much appreciate any help.

Thanks and regards

Martin