Re: NIS Elements software

Posted by Kurt Thorn on Dec 04, 2009; 11:11pm
URL: http://imagej.273.s1.nabble.com/NIS-Elements-software-tp3690220p3690222.html

I strongly suspect that this arises when you take 3 images.  
NIS-Elements saves them as a 3-channel tiff which is then interpreted by
ImageJ and other software packages as RGB tiff files.

We usually get around this by just saving each image as a separate tiff
file and then doing color overlays elsewhere or by just doing all
analysis in NIS-Elements.

Kurt

Martin Kann wrote:
> Hi everybody,
>
> we are having an issue with the Nikon NIS Elements software (basic research package, version 3.00 build 550) controlling our fluorescent microscope camera. This is what happens: when taking multichannel images the red and blue channel of a .tiff image are switched if one opens this image with any other software than NIS elements. When looking into the .tiff file metadata, it appears that the TRITC image plane has been assigned blue color, and the DAPI plane red. Of course there are workarounds by using ImageJ or batch processing of files with the channel-mixer in photoshop. However, it annoys me that I have to touch each and every file one more time than necessary for my own work and frequently explain the workarounds to other people using the microscope. Has anyone ever come across this problem and knows a way to fix this in NIS elements' preferences? Maybe somebody even has a small piece of code (either Mac or PC) or a photoshop droplet that automates the task?
> I would very much appreciate any help.
>
> Thanks and regards
>
> Martin
>