Sensitive Thresholding?

Posted by Wilson R S (AT) on Nov 30, 2009; 11:36am
URL: http://imagej.273.s1.nabble.com/Sensitive-Thresholding-tp3690288.html

I am trying to develop system whereby I can piece together in focus
segments from each colour image in a stack to a single image(like a
jigsaw puzzle if you will). The stack is of the same particle taken at
different Z values.

I have tried using the plugins "stack focuser" and "extended depth of
focus", however both result in a "fuzzy" image.

My initial idea was to use a thresholding method, however this seams to
pick out the out of focus sections first. I have tried a few different
thresholding methods, colour and greyscale (on green channel seems to be
the best), but nothing I get is useful.

 

Has anyone tried to create this sort of method before? Or is there a
plugin? Any help would be much appreciated.

I have available a sample stack of the sort of thing I am collecting, it
is only 5 images because of file attachment limits, generally I would be
using around 15 images.

 

Thanks In advance

 

Rhodri Wilson

F.A.T./HeSaS

University of Glamorgan

Wales

UK