Re: Sensitive Thresholding?

Posted by Przemko on Nov 30, 2009; 11:47am
URL: http://imagej.273.s1.nabble.com/Sensitive-Thresholding-tp3690288p3690289.html

Wilson R S (AT) wrote:

> I am trying to develop system whereby I can piece together in focus
> segments from each colour image in a stack to a single image(like a
> jigsaw puzzle if you will). The stack is of the same particle taken at
> different Z values.
>
> I have tried using the plugins "stack focuser" and "extended depth of
> focus", however both result in a "fuzzy" image.
>
> My initial idea was to use a thresholding method, however this seams to
> pick out the out of focus sections first. I have tried a few different
> thresholding methods, colour and greyscale (on green channel seems to be
> the best), but nothing I get is useful.
>
>  

Hi!
I posted on the same topic but got no reply. Basically, extended depth
of focus plugin failed me completely. I tried two different variants of
the plugin and played with the parameter. The images I got would range
from bad, through poor to disastrous. I tried Photoshop CS4 (the
previous ones do not have it) extended depth of focus tool and got a
great image the first time, and without fiddling. So my question, as a
follow up to yours, is why the extended depth of focus does not work in
ImageJ? Look at my previous post for more detail

Cheers!
Przemko


--
Przemko Tylzanowski PhD
Dept. Musculoskeletal Sciences
LSD & Joints
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Univeristy of Leuven
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